Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F21%3A00549488" target="_blank" >RIV/68081731:_____/21:00549488 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/21:00074767 RIV/00216224:14110/21:00121360
Result on the web
<a href="https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1" target="_blank" >https://link.springer.com/article/10.1140%2Fepjp%2Fs13360-021-01152-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1140/epjp/s13360-021-01152-1" target="_blank" >10.1140/epjp/s13360-021-01152-1</a>

Result language
angličtina
Original language name
Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers
Original language description
Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers-an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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