Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F22%3A00553555" target="_blank" >RIV/68081731:_____/22:00553555 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/22:00076328 RIV/00216224:14110/22:00125080 RIV/62157124:16170/22:43880478
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0003267021011181" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0003267021011181</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2021.339292" target="_blank" >10.1016/j.aca.2021.339292</a>
Alternative languages
Result language
angličtina
Original language name
Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections
Original language description
Urinary tract infections belong to the most common infections in the world. Besides communityacquired infections, nosocomial infections pose a high risk especially for patients having indwelling catheters, undergoing urological surgeries or staying at hospital for prolonged time. They can be often complicated by antimicrobial resistance and/or biofilm formation. Therefore, a rapid diagnostic tool enabling timely identification of a causative agent and its susceptibility to antimicrobials is a need. Raman spectroscopy appears to be a suitable method that allows rapid differentiation among microbes and provides a space for further analyses, such as determination of capability of biofilm formation or antimicrobial susceptibility/resistance in tested strains. Our work here presents a possibility to differ among most common microbes causing urinary tract infections (belonging to 20 species). We tested 254 strains directly from colonies grown on Mueller-Hinton agar plates. The results show that it is possible to distinguish among the tested species using Raman spectroscopy, which proves its great potential for future use in clinical diagnostics. Moreover, we present here a pilot study of a real-time analysis and identification (in less than 10 min) of single microbial cells directly in urine employing optical tweezers combined with Raman spectroscopy.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
1873-4324
Volume of the periodical
1191
Issue of the periodical within the volume
25 January
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
339292
UT code for WoS article
000744469700002
EID of the result in the Scopus database
2-s2.0-85120916888