Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F22%3A00565445" target="_blank" >RIV/68081731:_____/22:00565445 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/22:00077925 RIV/00216224:14110/22:00125725
Result on the web
<a href="https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcimb.2022.866463/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fcimb.2022.866463" target="_blank" >10.3389/fcimb.2022.866463</a>

Result language
angličtina
Original language name
Raman Spectroscopy - A Novel Method for Identification and Characterization of Microbes on a Single-Cell Level in Clinical Settings
Original language description
Rapid and accurate identification of pathogens causing infections is one of the biggest challenges in medicine. Timely identification of causative agents and their antimicrobial resistance profile can significantly improve the management of infection, lower costs for healthcare, mitigate ever-growing antimicrobial resistance and in many cases, save lives. Raman spectroscopy was shown to be a useful-quick, non-invasive, and non-destructivetool for identifying microbes from solid and liquid media. Modifications of Raman spectroscopy and/or pretreatment of samples allow single-cell analyses and identification of microbes from various samples. It was shown that those non-culture-based approaches could also detect antimicrobial resistance. Moreover, recent studies suggest that a combination of Raman spectroscopy with optical tweezers has the potential to identify microbes directly from human body fluids. This review aims to summarize recent advances in non-culture-based approaches of identification of microbes and their virulence factors, including antimicrobial resistance, using methods based on Raman spectroscopy in the context of possible use in the future point-of-care diagnostic process.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)

Project
<a href="/en/project/NU21-05-00341" target="_blank" >NU21-05-00341: Advanced methods in detection of pathogenic microorganisms and analysis the antimicrobials’ effect using Raman spectroscopy and other methods at the molecular level in optofluidic systems</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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