Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F23%3A00574278" target="_blank" >RIV/68081731:_____/23:00574278 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/23:00131292 RIV/00159816:_____/23:00078789

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233740/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233740/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2023.1233740" target="_blank" >10.3389/fmicb.2023.1233740</a>

Result language

angličtina

Original language name

Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity

Original language description

Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (PyoFun) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The PyoFun pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of PyoFun against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (PyoSyn). The results confirmed successful production of PyoFun in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of PyoFun, and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, PyoFun exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by PyoSyn. The findings of this study suggest that PyoFun has significant potential as a biological shield against harmful radiation. Notably, PyoFun is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes PyoFun a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NU21-05-00341" target="_blank" >NU21-05-00341: Advanced methods in detection of pathogenic microorganisms and analysis the antimicrobials’ effect using Raman spectroscopy and other methods at the molecular level in optofluidic systems</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

1664-302X

Volume of the periodical

14

Issue of the periodical within the volume

20 July

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

1233740

UT code for WoS article

001041737100001

EID of the result in the Scopus database

2-s2.0-85166760553