Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F23%3A00577394" target="_blank" >RIV/68081731:_____/23:00577394 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/23:00131818 RIV/00159816:_____/23:00079451

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2023.1272734" target="_blank" >10.3389/fmicb.2023.1272734</a>

Result language

angličtina

Original language name

Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis

Original language description

Introduction Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity.Methods In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress.Results We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (-20 degrees C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains.Conclusion The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NU21-05-00341" target="_blank" >NU21-05-00341: Advanced methods in detection of pathogenic microorganisms and analysis the antimicrobials’ effect using Raman spectroscopy and other methods at the molecular level in optofluidic systems</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

1664-302X

Volume of the periodical

14

Issue of the periodical within the volume

29 September

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

1272734

UT code for WoS article

001085828700001

EID of the result in the Scopus database

2-s2.0-85174573280