The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F25%3A00643166" target="_blank" >RIV/68081731:_____/25:00643166 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/26:0200061
Result on the web
<a href="https://link.springer.com/article/10.1007/s11274-025-04737-5" target="_blank" >https://link.springer.com/article/10.1007/s11274-025-04737-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11274-025-04737-5" target="_blank" >10.1007/s11274-025-04737-5</a>
Alternative languages
Result language
angličtina
Original language name
The characterization of halophilic polyhydroxyalkanoate-producing bacteria from brine in Solivar near Prešov (Slovakia)
Original language description
The present study aims to isolate and investigate temporal variability of the halophilic and halotolerant microbiota present in brine from former salt mine Solivar, Prešov (Slovakia) especially with respect to with their ability to produce polyhydroxyalkanoates (PHA). Brine sampling was performed in the year 2020 and 2021 and samples were inoculated on the R2A medium with 5% NaCl for the bacterial isolation. We obtained a total of 53 halophilic isolates and one halotolerant isolate, all of which were tested for their ability to produce PHA via Nile Blue A staining, Raman spectroscopy and Gas chromatography. The low diverse halophilic microbiota was dominated by Proteobacteria members (mainly Halomonas, Halovibrio, and Chromohalobacter sp.) and some of these bacteria represent newly identified taxa. Around 80% of the isolates were able to produce PHA during growth on glucose-rich media, which highlights the importance of PHA for adaptation to high-salinity environments. Poly(3-hydroxybutyrate) (PHB) was the main type of PHA produced with the yield up to 2.76 g/L in Halovibrio sp. HP20-59. Overall, our investigation pointed out that brine from Solivar shows genetically variable community of halophilic bacteria most of which are capable of accumulation of PHA, hereby confirming the high biotechnological potential of halophilic bacteria.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
World Journal of Microbiology and Biotechnology
ISSN
0959-3993
e-ISSN
1573-0972
Volume of the periodical
41
Issue of the periodical within the volume
12
Country of publishing house
DE - GERMANY
Number of pages
13
Pages from-to
505
UT code for WoS article
001637520000001
EID of the result in the Scopus database
2-s2.0-105024753372