Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00555569" target="_blank" >RIV/68081766:_____/22:00555569 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/22:43880362

Result on the web

<a href="https://actavet.vfu.cz/91/1/0059/" target="_blank" >https://actavet.vfu.cz/91/1/0059/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202291010059" target="_blank" >10.2754/avb202291010059</a>

Result language

angličtina

Original language name

Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

Original language description

Exposure to pollutants is considered one of the potential reasons of population declines in bats. In the context of previous studies, we managed to create and keep a wide collection of cell lines from European bat species. Liver cells were chosen for testing, as they represent the preferred model for toxicological studies. Bats are protected, cell lines replacing experimental animals thus represent a unique opportunity to examine effects of pollutants which animals are exposed to in their environments. Moreover, cell incubation temperature variation may simulate physiological states of heterothermic bats. Liver cell lines were cultivated to the required cell number. Exposure to five different concentrations of permethrin (PM) and imidacloprid (IMI) were used to determine cytotoxic effects of these pesticides on Nyctalus noctula-derived liver cells cultivated at 37 degrees C and 8 degrees C for 24 h. An assay based on the measurement of activity of lactate dehydrogenase released from damaged cells was used for quantitating cytotoxicity. Cytotoxicity of IMI ranged from 0% to 47% and from 56% to 67% at 37 degrees C and 8 degrees C, respectively. Cytotoxicity of PM ranged from 36% to 56% and from 43% to 88% at 37 degrees C and 8 degrees C, respectively. Permethrin was tested on the cells at an order of magnitude lower concentrations, even so, higher degree of cytotoxicity was recorded. Imidacloprid was more toxic to bat liver cells at a hibernation temperature than at body temperature of 37 degrees C.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Volume of the periodical

91

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

10

Pages from-to

59-68

UT code for WoS article

000752524500007

EID of the result in the Scopus database

2-s2.0-85124456600