Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00564481" target="_blank" >RIV/68081766:_____/22:00564481 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00127773
Result on the web
<a href="http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long" target="_blank" >http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1101/pdb.prot107739" target="_blank" >10.1101/pdb.prot107739</a>
Alternative languages
Result language
angličtina
Original language name
Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)
Original language description
Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cold Spring Harbor protocols
ISSN
1940-3402
e-ISSN
1559-6095
Volume of the periodical
2022
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
574-583
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85141888652