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Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00564481" target="_blank" >RIV/68081766:_____/22:00564481 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/22:00127773

  • Result on the web

    <a href="http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long" target="_blank" >http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1101/pdb.prot107739" target="_blank" >10.1101/pdb.prot107739</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)

  • Original language description

    Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10613 - Zoology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Cold Spring Harbor protocols

  • ISSN

    1940-3402

  • e-ISSN

    1559-6095

  • Volume of the periodical

    2022

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    574-583

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-85141888652