The opportunistic protist, Giardia intestinalis, occurs in gut-healthy humans in a high-income country
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F23%3A00578523" target="_blank" >RIV/68081766:_____/23:00578523 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/23:43907120
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2270077" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2270077</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/22221751.2023.2270077" target="_blank" >10.1080/22221751.2023.2270077</a>
Alternative languages
Result language
angličtina
Original language name
The opportunistic protist, Giardia intestinalis, occurs in gut-healthy humans in a high-income country
Original language description
Giardia intestinalis, a cosmopolitan gastrointestinal protist, is detected mainly in patients with clinical giardiasis in high-income countries. In contrast, there is very little information on the presence of Giardia in asymptomatic individuals. Therefore, the aim of this study was to determine the presence and prevalence of Giardia in gut-healthy volunteers in the Czech Republic and to perform a comparative evaluation of different diagnostic methods, since Giardia diagnostics is complicated. Our results confirmed that the qPCR method is the most sensitive method for detecting Giardia and revealed a prevalence of 7% (22/296) in asymptomatic individuals. In most cases, the colonization intensity ranged from 10-1-101. A conventional PCR protocol targeting the TPI gene was used to identify the assemblages. However, this protocol had limited sensitivity for Giardia amplification, effectively detecting colonization above an intensity of 104. In addition, Giardia was detected in 19% of the animals, which were closely associated with the study participants. However, due to methodological limitations, zoonotic transmission could not be clearly confirmed. Notably, contact with animals proved to be the only factor that had a significant impact on the incidence of Giardia in gut-healthy humans.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30302 - Epidemiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Emerging Microbes & Infections
ISSN
2222-1751
e-ISSN
2222-1751
Volume of the periodical
12
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
2270077
UT code for WoS article
001099095300001
EID of the result in the Scopus database
2-s2.0-85175245819