Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F13%3A00398237" target="_blank" >RIV/68378041:_____/13:00398237 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bbrc.2013.09.133" target="_blank" >http://dx.doi.org/10.1016/j.bbrc.2013.09.133</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbrc.2013.09.133" target="_blank" >10.1016/j.bbrc.2013.09.133</a>
Alternative languages
Result language
angličtina
Original language name
Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS
Original language description
ELISA is widely used for urinary 8-oxo-7.8-dihydro-2´-deoxyguanosine (8-oxodG) analysis. However, a lack of agreement between ELISA and chromatographic methods confines its application to the assessment of relative urinary 8-oxodG level. We investigatedvarious ELISA modifications, seeking optimal conditions that would yield a good agreement between ELISA and HPLC-MS/MS. It was found, that purification of urine by solid phase extraction, then incubation with the anti-8-oxodG antibody at 40 C overnight and subsequent normalization of 8-oxodG levels per urinary creatinine resulted in a near-perfect correlation and agreement in mean levels between ELISA and HPLC-MS/MS. Our data show that, after introduction of a simple modification, ELISA quantification urinary 8-oxodG substantially improves. This method represents a significant advance for the ELISA, and encouraging its use in more studies adding to our knowledge of the role of this biomarker of oxidative stress in health and disease.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical and Biophysical Research Communications
ISSN
0006-291X
e-ISSN
—
Volume of the periodical
440
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
725-730
UT code for WoS article
000326905800045
EID of the result in the Scopus database
—