Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F15%3A00449670" target="_blank" >RIV/68378041:_____/15:00449670 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.ceca.2014.11.003" target="_blank" >http://dx.doi.org/10.1016/j.ceca.2014.11.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ceca.2014.11.003" target="_blank" >10.1016/j.ceca.2014.11.003</a>

Result language

angličtina

Original language name

Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus

Original language description

The purpose of the present study was to uncover the structure and function of molecules related to the central osmoreceptorin the supraoptic nucleus (SON) of rats. For this purpose, we performed RT-PCR and immunohistochemistry for TRPV1-related molecules, and patch-clamp and imaging of the cytosolic Ca2+ concentration ([Ca2+]i) to measure responses to osmolality changes and TRPV-related drugs. Arginine vasopressin (AVP), synthesized in the SON is a key factor in systemic osmolality regulation. We foundthat AVP neurones in the SON possess functional full-length TRPV1. Moreover, differences between the responses to capsaicin or hyperosmolality obtained in rat SON neurones and those obtained from dorsal root ganglion neurones or TRPV1-expressing cells indicate that the osmoreceptor expressed in the SON may be a heteromultimer in which TRPV1 is co-assembled with some other, yet unidentified, molecules.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Calcium

ISSN

0143-4160

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

24-37

UT code for WoS article

000349574000003

EID of the result in the Scopus database

2-s2.0-84922644005