Cytotoxicity Induced by Nanoparticles - the Standardisation of Methods within the QualityNano EP7 Network

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F15%3A00459251" target="_blank" >RIV/68378041:_____/15:00459251 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cytotoxicity Induced by Nanoparticles - the Standardisation of Methods within the QualityNano EP7 Network

Original language description

The uniqueness and diversity of nanomaterials (NMs) raise questions regarding their safety, as well as about feasible ways to assess their potential toxicity. Despite intensive nanotoxicological research, it is impossible to keep pace with the rate of development and production of new NMs. Modifications of NMs’ size, shape, and numerous other characteristics are performed to reach desirable industrial properties. To be able to alter, or rather minimize, the toxicity of NMs in the same way, we have to understand which NMs properties govern particular toxic effects. Computational modeling relating the physico-chemical properties of NMs with their toxic effects holds great promise in this area; however, the lack of high-quality comparable experimental data of properly characterized NMs hampers the development of reliable models. In the present study, we evaluated the cytotoxic potential of a set of TiO2 nanomaterials differing in basic characteristics (crystal structure, size, shape) using three in vitro assays (MTS, WST-1, LDH) and three human cell lines (alveolar A549 cells and bronchial BEAS-2B, representing in vitro models for studying inhalation toxicity, and PMA-differentiated THP-1 as a model of macrophages). TiO2 is one of the most frequently used nanomaterials. Although inert in the bulk form, some TiO2 nanoforms have been reported to elicit a toxic response. Our study compares the cytotoxicity results of 14 nano TiO2 variants in relation to their physico-chemical properties. Moreover, the sensitivity of the employed testing methods and cell lines was compared. The main purpose of the study was to generate high-quality data for the computational modeling of cytotoxicity (nano-QSAR).

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LO1508" target="_blank" >LO1508: Genomics and proteomics in the study of mechanisms of biological effects of produced nanoparticles</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

From Functionalized Nanostructures Towards Engineered Macrostructures

ISBN

978-80-88113-20-1

Number of pages of the result

11

Pages from-to

24-34

Number of pages of the book

176

Publisher name

Epika

Place of publication

Jindřichův Hradec

UT code for WoS chapter

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