Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00459623" target="_blank" >RIV/68378041:_____/16:00459623 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10324842

Result on the web

<a href="http://dx.doi.org/10.1016/j.scr.2016.03.010" target="_blank" >http://dx.doi.org/10.1016/j.scr.2016.03.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scr.2016.03.010" target="_blank" >10.1016/j.scr.2016.03.010</a>

Result language

angličtina

Original language name

Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells.

Original language description

Mesenchymal stem cells from different tissue display similar cell surface characteristics based on their fibroblastic nature, but also exhibit differences in molecular phenotype, growth rate, and their ability to differentiate into various cell phenotypes. The mechanisms underlying these differences remain poorly understood. We analyzed Ca2+ signals and membrane properties in rat adipose-derived stromal cells (ADSCs) and bone marrow stromal cells (BMSCs) in basal conditions, and then following a switch into medium that contains factors known to modify their character. Modified ADSCs (mADSCs) expressed L-type Ca2+ channels whereas both Land P/Q- channels were operational in mBMSCs. Both mADSCs and mBMSCs possessed functional endoplasmic reticulum Ca2+ stores, expressed ryanodine receptor-1 and -3, and exhibited spontaneous [Ca2+]i oscillations. The mBMSCs expressed P2X7 purinoceptors; the mADSCs expressed both P2X (but not P2X7) and P2Y (but not P2Y1) receptors. Both types of stromal cells exhibited [Ca2+]i responses to vasopressin (AVP) and expressed V1 type receptors. Functional oxytocin (OT) receptors were, in contrast, expressed only in modified ADSCs and BMSCs. Electrophysiological data revealed that passive ion currents dominated the membrane conductance in ADSCs and BMSCs. Medium modification led to a significant shift in the reversal potential of passive currents from40 to50 mV in cells in basal to80 mV in modified cells. Hence membrane conductance was mediated by non-selective channels in cells in basal conditions, whereas in modified medium conditions, it was associated with K+-selective channels. Our results indicate that modification of ADSCs and BMSCs by alteration in medium formulation is associated with significant changes in their Ca2+ signaling and membrane properties.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Stem Cell Research

ISSN

1873-5061

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

3

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

622-634

UT code for WoS article

000377416900012

EID of the result in the Scopus database

2-s2.0-84962360781