Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F16%3A00460262" target="_blank" >RIV/68378041:_____/16:00460262 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10324778 RIV/00216208:11130/16:10324778
Result on the web
<a href="http://dx.doi.org/10.1016/j.exer.2016.04.016" target="_blank" >http://dx.doi.org/10.1016/j.exer.2016.04.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exer.2016.04.016" target="_blank" >10.1016/j.exer.2016.04.016</a>
Alternative languages
Result language
angličtina
Original language name
Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy.
Original language description
Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated gamma-Fe2O3) and studied their effect on proliferation and neuronal differentiation. nnMaterials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR. nnResults: Cell proliferation was not affected by PLL-coated gamma-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated gamma-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles. nnConclusion: Our results show that cells labeled with PLL-coated gamma-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental Eye Research
ISSN
0014-4835
e-ISSN
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Volume of the periodical
147
Issue of the periodical within the volume
may.
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
128-137
UT code for WoS article
000378186800015
EID of the result in the Scopus database
2-s2.0-84969267999