A local application of mesenchymal stem cells and cyclosporine A attenuates immune response by a switch in macrophage phenotype

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00454064" target="_blank" >RIV/68378041:_____/17:00454064 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10320407

Result on the web

<a href="http://dx.doi.org/10.1002/term.2044" target="_blank" >http://dx.doi.org/10.1002/term.2044</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/term.2044" target="_blank" >10.1002/term.2044</a>

Result language

angličtina

Original language name

A local application of mesenchymal stem cells and cyclosporine A attenuates immune response by a switch in macrophage phenotype

Original language description

The immunosuppressive effects of systemically administered mesenchymal stem cells (MSCs) and immunosuppressive drugs have been well documented. We analysed the mechanisms underlying the therapeutic effect of MSCs applied locally in combination with non-specific immunosuppression in a mouse model of allogeneic skin transplantation. The MSC-seeded and cyclosporine A (CsA)-loaded nanofibre scaffolds were applied topically to skin allografts in a mouse model and the local immune response was assessed and characterized. MSCs migrated from the scaffold into the side of injury and were detected in the graft region and draining lymph nodes (DLNs). The numbers of graft-infiltrating macrophages and the production of nitric oxide (NO) were significantly decreased in recipients treated with MSCs and CsA, and this reduction correlated with impaired production of IFN. in the graft and DLNs. In contrast, the proportion of alternatively activated macrophages (F4/80(+)CD206(+) cells) and the production of IL-10 by intragraft macrophages were significantly upregulated. The ability of MSCs to alter the phenotype of macrophages from the M1 type into an M2 population was confirmed in a co-culture system in vitro. We suggest that the topical application of MSCs in combination with CsA induces a switch in macrophages to a population with an alternatively activated 'healing' phenotype and producing elevated levels of IL-10. These alterations in macrophage phenotype and function could represent one of the mechanisms of immunosuppressive action of MSCs applied in combination with CsA.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Tissue Engineering and Regenerative Medicine

ISSN

1932-6254

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1456-1465

UT code for WoS article

000402987500013

EID of the result in the Scopus database

2-s2.0-84933574461