Cyclosporine A promotes the therapeutic effect of mesenchymal stem cells on transplantation reaction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00517905" target="_blank" >RIV/68378041:_____/19:00517905 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/19:00523651

Result on the web

<a href="https://portlandpress.com/clinsci/article-abstract/133/21/2143/220846/Cyclosporine-A-promotes-the-therapeutic-effect-of?redirectedFrom=fulltext" target="_blank" >https://portlandpress.com/clinsci/article-abstract/133/21/2143/220846/Cyclosporine-A-promotes-the-therapeutic-effect-of?redirectedFrom=fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1042/CS20190294" target="_blank" >10.1042/CS20190294</a>

Result language

angličtina

Original language name

Cyclosporine A promotes the therapeutic effect of mesenchymal stem cells on transplantation reaction

Original language description

The successful application of mesenchymal stem cells (MSCs) remains a major challenge in stem cell therapy. Currently, several in vitro studies have indicated potentially beneficial interactions of MSCs with immunosuppressive drugs. These interactions can be even more complex in vivo, and it is in this setting that we investigate the effect of MSCs in combination with Cyclosporine A (CsA) on transplantation reaction and allogeneic cell survival. Using an in vivo mouse model, we found that CsA significantly promoted the survival of MSCs in various organs and tissues of the recipients. In addition, compared to treatment with CsA or MSCs alone, the survival of transplanted allogeneic cells was significantly improved after the combined application of MSCs with CsA. We further observed that the combinatory treatment suppressed immune response to the alloantigen challenge and modulated the immune balance by harnessing proinflammatory CD4+T-bet+ and CD4+RORγt+ cell subsets. These changes were accompanied by a significant decrease in IL-17 production along with an elevated level of IL-10. Co-cultivation of purified naive CD4+ cells with peritoneal macrophages isolated from mice treated with MSCs and CsA revealed that MSC-educated macrophages play an important role in the immunomodulatory effect observed on distinct T-cell subpopulations. Taken together, our findings suggest that CsA promotes MSC survival in vivo and that the therapeutic efficacy of the combination of MSCs with CsA is superior to each monotherapy. This combinatory treatment thus represents a promising approach to reducing immunosuppressant dosage while maintaining or even improving the outcome of therapy.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30213 - Transplantation

Project

<a href="/en/project/GA19-02290S" target="_blank" >GA19-02290S: The use of stem cells for induction of specific transplantation tolerance</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical Science

ISSN

1470-8736

e-ISSN

—

Volume of the periodical

133

Issue of the periodical within the volume

21

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

2143-2157

UT code for WoS article

000511197800002

EID of the result in the Scopus database

2-s2.0-85074676167