A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00483105" target="_blank" >RIV/68378041:_____/17:00483105 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/17:10373874 RIV/68407700:21220/17:00317963 RIV/68407700:21460/17:00317963 RIV/68407700:21720/17:00317963 RIV/00216208:11310/17:10373874

Result on the web

<a href="http://dx.doi.org/10.1039/c7ra08728d" target="_blank" >http://dx.doi.org/10.1039/c7ra08728d</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c7ra08728d" target="_blank" >10.1039/c7ra08728d</a>

Result language

angličtina

Original language name

A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells

Original language description

Among the main aims of tissue engineering certainly belong actively acting scaffolds with a controlled release of bioactivemolecules. This is important for cell-free scaffolds in regenerativemedicine. The scaffold topology is crucial for cell-scaffold interactions and plays a pivotal role in stimulation of cell adhesion and proliferation through affecting cell morphology and intercellular contacts. The aim of this study was to characterise proliferation of different skin cells on core-shell 2D and 3D nano-and microfibre scaffolds from poly-3caprolactone loaded with lyophilised platelets. The electrospinning technique forms dense fibrous 2D scaffolds with limited cell infiltration, whereas the centrifugal spinning enables deep cell penetration due to its open 3D structure. The core of the prepared fibres was loaded with lyophilised platelet fraction and its release was controlled by the Pluronic F-68 concentration. This resulted in the preparation of functionalized scaffolds with a tuneable sustained release lasting more than 30 days. Two dermal cell lines, keratinocytes and fibroblasts, were grown on these functionalized scaffolds. While keratinocytes, epithelial cells, proliferated significantly better on the 2D structure with optimal stimulation of cell proliferation on the scaffolds containing 5% PF-68, fibroblasts proliferated well both on the 2D and 3D scaffolds but with a higher initial adhesion on the 3D forcespun fibre scaffold. Furthermore, a dose-dependent stimulation of proliferation by the released platelet lyophilisate was shown. We have concluded that beside the scaffold composition and its functionalization with bioactive molecules, the scaffold structure plays a significant role in regenerative medicine and dermal tissue engineering.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

RSC Advances

ISSN

2046-2069

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

85

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

53706-53719

UT code for WoS article

000416831000007

EID of the result in the Scopus database

2-s2.0-85035323539