EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00487517" target="_blank" >RIV/68378041:_____/17:00487517 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1242/jcs.207423" target="_blank" >http://dx.doi.org/10.1242/jcs.207423</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jcs.207423" target="_blank" >10.1242/jcs.207423</a>

Result language

angličtina

Original language name

EFA6 regulates selective polarised transport and axon regeneration from the axon initial segment

Original language description

Central nervous system (CNS) axons lose their intrinsic ability to regenerate upon maturity, whereas peripheral nervous system (PNS) axons do not. A key difference between these neuronal types is their ability to transport integrins into axons. Integrins can mediate PNS regeneration, but are excluded from adult CNS axons along with their Rab11 carriers. We reasoned that exclusion of the contents of Rab11 vesicles including integrins might contribute to the intrinsic inability of CNS neurons to regenerate, and investigated this by performing laser axotomy. We identify a novel regulator of selective axon transport and regeneration, the ARF6 guanine-nucleotide-exchange factor (GEF) EFA6 (also known as PSD). EFA6 exerts its effects from a location within the axon initial segment (AIS). EFA6 does not localise at the AIS in dorsal root ganglion (DRG) axons, and in these neurons, ARF6 activation is counteracted by an ARF GTPase-activating protein (GAP), which is absent from the CNS, ACAP1. Depleting EFA6 from cortical neurons permits endosomal integrin transport and enhances regeneration, whereas overexpressing EFA6 prevents DRG regeneration. Our results demonstrate that ARF6 is an intrinsic regulator of regenerative capacity, implicating EFA6 as a focal molecule linking the AIS, signalling and transport. n

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

—

Volume of the periodical

130

Issue of the periodical within the volume

21

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

3663-3675

UT code for WoS article

000414149400009

EID of the result in the Scopus database

2-s2.0-85032824407