Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00502519" target="_blank" >RIV/68378041:_____/19:00502519 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/19:00502519 RIV/67985858:_____/19:00502519 RIV/67985904:_____/19:00502519 RIV/00027162:_____/19:N0000246 and 3 more
Result on the web
<a href="https://academic.oup.com/toxsci/article/168/1/190/5220776" target="_blank" >https://academic.oup.com/toxsci/article/168/1/190/5220776</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/toxsci/kfy288" target="_blank" >10.1093/toxsci/kfy288</a>
Alternative languages
Result language
angličtina
Original language name
Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice
Original language description
Despite the wide application of nanomaterials, toxicity studies of nanoparticles (NP) are often limited to in vitro cell models, and the biological impact of NP exposure in mammals has not been thoroughly investigated. Zinc oxide (ZnO) NPs are commonly used in various consumer products. To evaluate the effects of the inhalation of ZnO NP in mice, we studied splice junction expression in the lungs as a proxy to gene expression changes analysis. Female ICR mice were treated with 6.46 × 104 and 1.93 × 106 NP/cm3 for 3 days and 3 months, respectively. An analysis of differential expression and alternative splicing events in 298 targets (splice junctions) of 68 genes involved in the processes relevant to the biological effects of ZnO NP was conducted using next-generation sequencing. Three days of exposure resulted in the upregulation of IL-6 and downregulation of BID, GSR, NF-kB2, PTGS2, SLC11A2, and TXNRD1 splice junction expression, 3 months of exposure increased the expression of splice junctions in ALDH3A1, APAF1, BID, CASP3, DHCR7, GCLC, GCLM, GSR, GSS, EHHADH, FAS, HMOX-1, IFNγ, NF-kB1, NQO-1, PTGS1, PTGS2, RAD51, RIPK2, SRXN1, TRAF6, and TXNRD1. Alternative splicing of TRAF6 and TXNRD1 was induced after 3 days of exposure to 1.93 × 106 NP/cm3. In summary, we observed changes of splice junction expression in genes involved in oxidative stress, apoptosis, immune response, inflammation, and DNA repair, as well as the induction of alternative splicing in genes associated with oxidative stress and inflammation. Our data indicate the potential negative biological effects of ZnO NP inhalation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Toxicological Sciences
ISSN
1096-6080
e-ISSN
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Volume of the periodical
168
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
190-200
UT code for WoS article
000462865100017
EID of the result in the Scopus database
2-s2.0-85062183828