Two-tailed RT-qPCR panel for quality control of circulating microRNA studies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00505062" target="_blank" >RIV/68378041:_____/19:00505062 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/19:00505062 RIV/00216208:11310/19:10406756 RIV/61989592:15310/19:73597159
Result on the web
<a href="https://www.nature.com/articles/s41598-019-40513-w" target="_blank" >https://www.nature.com/articles/s41598-019-40513-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-40513-w" target="_blank" >10.1038/s41598-019-40513-w</a>
Alternative languages
Result language
angličtina
Original language name
Two-tailed RT-qPCR panel for quality control of circulating microRNA studies
Original language description
Circulating cell-free microRNAs are promising candidates for minimally invasive clinical biomarkers for the diagnosis, prognosis and monitoring of many human diseases. Despite substantial efforts invested in the field, the research so far has failed to deliver expected results. One of the contributing factors is general lack of agreement between various studies, partly due to the considerable technical challenges accompanying the workflow. Pre-analytical variables including sample collection, RNA isolation, and quantification are sources of bias that may hamper biological interpretation of the results. Here, we present a Two-tailed RT-qPCR panel for quality control, monitoring of technical performance, and optimization of microRNA profiling experiments from biofluid samples. The Two-tailed QC (quality control) panel is based on two sets of synthetic spike-in molecules and three endogenous microRNAs that are quantified with the highly specific Two-tailed RT-qPCR technology. The QC panel is a cost-effective way to assess quality of isolated microRNA, degree of inhibition, and erythrocyte contamination to ensure technical soundness of the obtained results. We provide assay sequences, detailed experimental protocol and guide to data interpretation. The application of the QC panel is demonstrated on the optimization of RNA isolation from biofluids with the miRNeasy Serum/Plasma Advanced Kit (Qiagen).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
MAR 12 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
4255
UT code for WoS article
000460922200091
EID of the result in the Scopus database
2-s2.0-85062845745