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The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00518846" target="_blank" >RIV/68378041:_____/19:00518846 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26620/19:PU130004 RIV/00216224:14310/19:00108936

  • Result on the web

    <a href="https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12771" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12771</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1111/jmi.12771" target="_blank" >10.1111/jmi.12771</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

  • Original language description

    This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent - heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30404 - Biomaterials (as related to medical implants, devices, sensors)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Microscopy

  • ISSN

    0022-2720

  • e-ISSN

  • Volume of the periodical

    273

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

    169-177

  • UT code for WoS article

    000458426100002

  • EID of the result in the Scopus database

    2-s2.0-85057052831