The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU130004" target="_blank" >RIV/00216305:26620/19:PU130004 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/19:00518846 RIV/00216224:14310/19:00108936
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/jmi.12771" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/jmi.12771</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jmi.12771" target="_blank" >10.1111/jmi.12771</a>

Result language
angličtina
Original language name
The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
Original language description
This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21101 - Food and beverages

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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