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Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F20%3A00539261" target="_blank" >RIV/68378041:_____/20:00539261 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.nature.com/articles/s41596-020-0398-1" target="_blank" >https://www.nature.com/articles/s41596-020-0398-1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41596-020-0398-1" target="_blank" >10.1038/s41596-020-0398-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

  • Original language description

    The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between desirable and essential information: essential information refers to the precise details that are necessary to assess the quality of the experimental work, whereas desirable information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.nnHere, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Nature Protocols

  • ISSN

    1754-2189

  • e-ISSN

  • Volume of the periodical

    15

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    10

  • Pages from-to

    3817-3826

  • UT code for WoS article

    000583953500001

  • EID of the result in the Scopus database

    2-s2.0-85093963487