Measuring DNA modifications with the comet assay: a compendium of protocols
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F23%3A00580560" target="_blank" >RIV/68378041:_____/23:00580560 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/23:10457902
Result on the web
<a href="https://www.nature.com/articles/s41596-022-00754-y" target="_blank" >https://www.nature.com/articles/s41596-022-00754-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41596-022-00754-y" target="_blank" >10.1038/s41596-022-00754-y</a>
Alternative languages
Result language
angličtina
Original language name
Measuring DNA modifications with the comet assay: a compendium of protocols
Original language description
The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility.nnThe comet assay is commonly used to assess DNA damage. This collection of consensus protocols includes adaptations for a wide range of species and sample types, assay formats and detection of different types of DNA lesions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Protocols
ISSN
1754-2189
e-ISSN
1750-2799
Volume of the periodical
18
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
61
Pages from-to
929-989
UT code for WoS article
000921193000001
EID of the result in the Scopus database
2-s2.0-85146929563