Functionalization of polymeric nanofibers using platelets for melanocyte culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F20%3A00539763" target="_blank" >RIV/68378041:_____/20:00539763 - isvavai.cz</a>

Result on the web

<a href="https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/6131" target="_blank" >https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/6131</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14311/CTJ.2020.1.03" target="_blank" >10.14311/CTJ.2020.1.03</a>

Result language

angličtina

Original language name

Functionalization of polymeric nanofibers using platelets for melanocyte culture

Original language description

Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture.

Czech name

—

Czech description

—

Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

30404 - Biomaterials (as related to medical implants, devices, sensors)

Project

<a href="/en/project/LO1508" target="_blank" >LO1508: Genomics and proteomics in the study of mechanisms of biological effects of produced nanoparticles</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Lékař a technika. Biomedicinské inženýrství a informatika

ISSN

0301-5491

e-ISSN

—

Volume of the periodical

50

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

16-22

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85092597508