Antiapoptotic Properties of Mesenchymal Stem Cells in a Mouse Model of Corneal Inflammation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F21%3A00551750" target="_blank" >RIV/68378041:_____/21:00551750 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10436886
Result on the web
<a href="https://www.liebertpub.com/doi/10.1089/scd.2020.0195" target="_blank" >https://www.liebertpub.com/doi/10.1089/scd.2020.0195</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/scd.2020.0195" target="_blank" >10.1089/scd.2020.0195</a>
Alternative languages
Result language
angličtina
Original language name
Antiapoptotic Properties of Mesenchymal Stem Cells in a Mouse Model of Corneal Inflammation
Original language description
Mesenchymal stem cells (MSCs) represent a population of adult stem cells that have potent immunoregulatory, anti-inflammatory, and antiapoptotic properties. In addition, they have ability to migrate to the site of inflammation or injury, where they contribute to the regeneration and healing process. For these properties, MSCs have been used as therapeutic cells in several models, including treatment of damages or disorders of the ocular surface. If the damage of the ocular surface is extensive and involves a limbal region where limbal stem cell reside, MSC therapy has been proved as the effective treatment approach. Although the anti-inflammatory properties of MSCs have been well characterized, mechanisms of antiapoptotic action of MSCs are not well recognized. Using a chemically damaged cornea in a mouse model, we showed that the injury decreases expression of the gene for antiapoptotic molecule Bcl-2 and increases the expression of proapoptotic genes Bax and p53. These changes were attenuated by local transplantation of MSCs after corneal damage. The antiapoptotic effect of MSCs was tested in an in vitro model of co-cultivation of corneal explants with MSCs. The apoptosis of corneal cells in the explants was induced by proinflammatory cytokines and was significantly inhibited in the presence of MSCs. The antiapoptotic effect of MSCs was mediated by paracrine action, as confirmed by separation of the explants in inserts or by supernatants from MSCs. In addition, MSCs decreased the expression of genes for the molecules associated with endoplasmic reticulum stress Atf4, Bip, and p21, which are associated with apoptosis. The results show that MSCs inhibit the expression of proapoptotic genes and decrease the number of apoptotic cells in the damaged corneas, and this action might be one of the mechanisms of the therapeutic action of MSCs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
<a href="/en/project/GA19-02290S" target="_blank" >GA19-02290S: The use of stem cells for induction of specific transplantation tolerance</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem Cells and Development
ISSN
1547-3287
e-ISSN
1557-8534
Volume of the periodical
30
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
418-427
UT code for WoS article
000634637600001
EID of the result in the Scopus database
2-s2.0-85104172714