Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F21%3A00552881" target="_blank" >RIV/68378041:_____/21:00552881 - isvavai.cz</a>
Alternative codes found
RIV/00027014:_____/21:N0000038
Result on the web
<a href="https://www.mdpi.com/2076-2615/11/5/1250" target="_blank" >https://www.mdpi.com/2076-2615/11/5/1250</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ani11051250" target="_blank" >10.3390/ani11051250</a>
Alternative languages
Result language
angličtina
Original language name
Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report
Original language description
The long-term storage of biological material (sperm, oocytes, embryos, etc.) is essential not only for animal breeding programs, human ART, and basic biology, but also for rescuing endangered animal species and other technologies. Whilst sperm storage is almost perfected for some species (bovine, human, mouse), many problems remain in others. In our contribution, we present a simple approach that can be used for the rapid evaluation of DNA damage level in sperm nuclei after freezing. This approach can be useful especially in those cases when the amount of frozen biological material is limited and permits much higher flexibility when modifying chosen preservation approaches.nnXenogenic mammalian sperm heads injected into mouse ovulated oocytes decondense and form pronuclei in which sperm DNA parameters can be evaluated. We suggest that this approach can be used for the assessment of sperm DNA damage level and the evaluation of how certain sperm treatments (freezing, lyophilization, etc.) influence the quality of spermatozoa.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animals
ISSN
2076-2615
e-ISSN
2076-2615
Volume of the periodical
11
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
7
Pages from-to
1250
UT code for WoS article
000653360500001
EID of the result in the Scopus database
2-s2.0-85104581810