Understanding the Biological Basis of Glioblastoma Patient-derived Spheroids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F21%3A00553042" target="_blank" >RIV/68378041:_____/21:00553042 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/21:10426088 RIV/61383082:_____/21:00001019 RIV/00216208:11110/21:10426088 RIV/00179906:_____/21:10426088 RIV/00216208:11130/21:10426088
Result on the web
<a href="https://ar.iiarjournals.org/content/41/3/1183" target="_blank" >https://ar.iiarjournals.org/content/41/3/1183</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.21873/anticanres.14875" target="_blank" >10.21873/anticanres.14875</a>
Alternative languages
Result language
angličtina
Original language name
Understanding the Biological Basis of Glioblastoma Patient-derived Spheroids
Original language description
Background/Aim: Resistance to glioblastoma (GB) therapy is attributed to the presence of glioblastoma stem cells (GSC). Here, we defined the behavior of GSC as it pertains to proliferation, migration, and angiogenesis. Materials and Methods: Human-derived GSC were isolated and cultured from GB patient tumors. Xenograft GSC were extracted from the xenograft tumors, and spheroids were created and compared with human GSC spheroids by flow cytometry, migration, proliferation, and angiogenesis assays. Oct3/4 and Sox2, GFAP, and Ku80 expression was assessed by immunoanalysis. Results: The xenograft model showed the formation of two different tumors with distinct characteristics. Tumors formed at 2 weeks were less aggressive with well-defined margins, whereas tumors formed in 5 months were diffuse and aggressive. Expression of Oct3/4 and Sox2 was positive in both human and xenograft GSC. Positive Ku80 expression in xenograft GSC confirmed their human origin. Human and xenograft GSC migrated vigorously in collagen and Matrigel, respectively. Xenograft GSC displayed a higher rate of migration and invasion than human GSC. Conclusion: Human GSC were more aggressive in growth and proliferation than xenograft GSC, while xenograft GSC had increased invasion and migration compared to human GSC. A simple in vitro spheroid system for GSC provides a superior platform for the development of precision medicine in the treatment of GB.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
<a href="/en/project/EF15_003%2F0000419" target="_blank" >EF15_003/0000419: Center of Reconstructive Neuroscience</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anticancer Research
ISSN
0250-7005
e-ISSN
1791-7530
Volume of the periodical
41
Issue of the periodical within the volume
3
Country of publishing house
GR - GREECE
Number of pages
13
Pages from-to
1183-1195
UT code for WoS article
000632017100006
EID of the result in the Scopus database
2-s2.0-85103196225