The Effect of Alendronate on Osteoclastogenesis in Different Combinations of M-CSF and RANKL Growth Factors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F21%3A00553161" target="_blank" >RIV/68378041:_____/21:00553161 - isvavai.cz</a>
Alternative codes found
RIV/46747885:24510/21:00009622
Result on the web
<a href="https://www.mdpi.com/2218-273X/11/3/438" target="_blank" >https://www.mdpi.com/2218-273X/11/3/438</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biom11030438" target="_blank" >10.3390/biom11030438</a>
Alternative languages
Result language
angličtina
Original language name
The Effect of Alendronate on Osteoclastogenesis in Different Combinations of M-CSF and RANKL Growth Factors
Original language description
Bisphosphonates (BPs) are compounds resembling the pyrophosphate structure. BPs bind the mineral component of bones. During the bone resorption by osteoclasts, nitrogen-containing BPs are released and internalized, causing an inhibition of the mevalonate pathway. As a consequence, osteoclasts are unable to execute their function. Alendronate (ALN) is a bisphosphonate used to treat osteoporosis. Its administration could be associated with adverse effects. The purpose of this study is to evaluate four different ALN concentrations, ranging from 10(-6) to 10(-10) M, in the presence of different combinations of M-CSF and RANKL, to find out the effect of low ALN concentrations on osteoclastogenesis using rat and human peripheral blood mononuclear cells. The cytotoxic effect of ALN was evaluated based on metabolic activity and DNA concentration measurement. The alteration in osteoclastogenesis was assessed by the activity of carbonic anhydrase II (CA II), tartrate-resistant acid phosphatase staining, and actin ring formation. The ALN concentration of 10(-6) M was cytotoxic. Low ALN concentrations of 10(-8) and 10(-10) M promoted proliferation, osteoclast-like cell formation, and CA II activity. The results indicated the induction of osteoclastogenesis with low ALN concentrations. However, when high doses of ALN were administered, their cytotoxic effect was demonstrated.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/GA18-09306S" target="_blank" >GA18-09306S: Development of advanced 3D in vitro model of osteoporosis and investigation of the mechanism of biomaterials osteointegration for bone regeneration</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomolecules
ISSN
2218-273X
e-ISSN
2218-273X
Volume of the periodical
11
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
438
UT code for WoS article
000633399300001
EID of the result in the Scopus database
2-s2.0-85102594658