Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F24%3A00581742" target="_blank" >RIV/68378041:_____/24:00581742 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0887233323001595?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0887233323001595?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tiv.2023.105710" target="_blank" >10.1016/j.tiv.2023.105710</a>
Alternative languages
Result language
angličtina
Original language name
Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
Original language description
DNA damage can impair normal cellular functions and result in various pathophysiological processes including cardiovascular diseases and cancer. We compared the genotoxic potential of diverse DNA damaging agents, and focused on their effects on the DNA damage response (DDR) and cell fate in human lung cells BEAS-2B. Poly-cyclic aromatic hydrocarbons [PAHs, benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP)] induced DNA strand breaks and oxidative damage to DNA, anticancer drugs doxorubicin (DOX) and 5-bromo-2 '-deoxyuridine (BrdU) were less effective. DOX triggered the most robust p53 signaling indicating activation of DDR, followed by cell cycle arrest in the G2/M phase, induction of apoptosis and senescence, possibly due to the severe and irreparable DNA lesions. BrdU not only activated p53, but also increased the percentage of G1-phased cells and caused a massive accumulation of senescent cells. In contrast, regardless the activation of p53, both PAHs did not substantially affect the cell cycle distribution or senescence. Finally, a small fraction of cells accumulated only in the G2/M phase and exhibited increased cell death after the prolonged incubation with B[a]P. Overall, we characterized differential responses to diverse DNA damaging agents resulting in specific cell fate and highlighted the key role of DNA lesion type and the p53 signaling persistence.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30304 - Public and environmental health
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Toxicology in Vitro
ISSN
0887-2333
e-ISSN
1879-3177
Volume of the periodical
94
Issue of the periodical within the volume
February
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
105710
UT code for WoS article
001100361700001
EID of the result in the Scopus database
2-s2.0-85173992366