The Immunomodulatory Effect of Silver Nanoparticles in a Retinal Infammatory Environment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F24%3A00603462" target="_blank" >RIV/68378041:_____/24:00603462 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s10753-024-02128-w" target="_blank" >https://link.springer.com/article/10.1007/s10753-024-02128-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10753-024-02128-w" target="_blank" >10.1007/s10753-024-02128-w</a>

Result language

angličtina

Original language name

The Immunomodulatory Effect of Silver Nanoparticles in a Retinal Infammatory Environment

Original language description

Activation of immune response plays an important role in the development of retinal diseases. One of the main populations of immune cells contributing to the retinal homeostasis are microglia, which represent a population of residential macrophages. However, under pathological conditions, microglia become activated and rather support a harmful inflammatory reaction and retinal angiogenesis. Therefore, targeting these cells could provide protection against retinal neuroinflammation and neovascularization. In the recent study, we analyzed effects of silver nanoparticles (AgNPs) on microglia in vitro and in vivo. We showed that the AgNPs interact in vitro with stimulated mouse CD45/CD11b positive cells (microglia/macrophages), decrease their secretion of nitric oxide and vascular endothelial growth factor, and regulate the expression of genes for Iba-1 and interleukin-1β (IL-1β). In our in vivo experimental mouse model, the intravitreal application of a mixture of proinflammatory cytokines tumor necrosis factor-α, IL-1β and interferon-γ induced local inflammation and increased local expression of genes for inducible nitric oxide synthase, IL-α, IL-1β and galectin-3 in the retina. This stimulation of local inflammatory reaction was significantly inhibited by intravitreal administration of AgNPs. The application of AgNPs also decreased the presence of CD11b/Galectin-3 positive cells in neuroinflammatory retina, but did not influence viability of cells and expression of gene for rhodopsin in the retinal tissue. These data indicate that AgNPs regulate reactivity of activated microglia in the diseased retina and thus could provide a beneficial effect for the treatment of several retinal diseases.

Czech name

—

Czech description

—

Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Inflammation

ISSN

0360-3997

e-ISSN

1573-2576

Volume of the periodical

neuveden

Issue of the periodical within the volume

August

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85202192272