Combined strategy of α 9-integrin transduction and AEIDGIEL peptide-functionalized fibrin gel biomaterials to promote mature DRG neurite growth
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00618542" target="_blank" >RIV/68378041:_____/25:00618542 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/25:10497322
Result on the web
<a href="https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2025.1568004/full" target="_blank" >https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2025.1568004/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fncel.2025.1568004" target="_blank" >10.3389/fncel.2025.1568004</a>
Alternative languages
Result language
angličtina
Original language name
Combined strategy of α 9-integrin transduction and AEIDGIEL peptide-functionalized fibrin gel biomaterials to promote mature DRG neurite growth
Original language description
Introduction: Spinal cord injury involves complex pathobiological mechanisms, necessitating a multidimensional approach for its cure. Previous studies have shown that α9-integrin expression and activation in mature dorsal root ganglion neurons enable the regeneration of injured axons within the spinal cord. However, tissue cavitation and fibrosis impede the regenerating axons from following their usual pathways, forcing them to seek alternative routes rich in tenascin-C, the primary ligand of the integrin. Fibrin gel, an FDA-approved and biocompatible material, can offer three-dimensional support for axonal extension through the cavitated area, thus preventing the formation of aberrant paths and connections that occur in the absence of a suitable scaffold.nMethods: The aim of this study was to investigate how combining α9-integrin expression by adeno-associated virus with the use of a fibrin gel as an extracellular microenvironment affects the growth of mature DRG neurites in vitro. Additionally, we sought to functionalize fibrin with integrin ligand peptides, specifically AEIDGIEL, the active domain of tenascin-C, to ensure α9-integrin activation.nResults: Our results indicate that fibrin gels are a suitable biomaterial for promoting neurite growth and that AEIDGIEL peptide effectively activates the integrin. Furthermore, we corroborate an autocrine signaling loop of α9-integrin and TN-C produced by neurons.nDiscussion: the proposed combination therapy of α9-integrin and fibrin gel biomaterials incorporating AEIDGIEL peptide shows promise for addressing the complex challenges of spinal cord injury and promoting effective neural regeneration, laying the foundation for further in vivo research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30103 - Neurosciences (including psychophysiology)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Cellular Neuroscience
ISSN
1662-5102
e-ISSN
1662-5102
Volume of the periodical
19
Issue of the periodical within the volume
April
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
1568004
UT code for WoS article
001466513100001
EID of the result in the Scopus database
2-s2.0-105002614443