Protamine sequence determines species-specific nuclear shape and histone retention
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00637862" target="_blank" >RIV/68378041:_____/25:00637862 - isvavai.cz</a>
Alternative codes found
RIV/00027014:_____/25:10006339
Result on the web
<a href="https://www.cell.com/iscience/fulltext/S2589-0042(25)01363-X" target="_blank" >https://www.cell.com/iscience/fulltext/S2589-0042(25)01363-X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.isci.2025.113102" target="_blank" >10.1016/j.isci.2025.113102</a>
Alternative languages
Result language
angličtina
Original language name
Protamine sequence determines species-specific nuclear shape and histone retention
Original language description
The nuclear shape observed after the forced expression of mouse or human protamine 1 (PRM1) in fibroblasts led us to propose the hypothesis that the PRM1 sequence plays an important role in imposing the overall shape of the protaminized nucleus. Comparison of mouse and human PRM1 sequences pointed to cysteines 15 and 29 as potential critical residues in the mouse PRM1 sequence, inducing the characteristic mouse “hooked” nuclear sperm shape. To explore this idea, mice with mutations in PRM1 Cys15 and Cys29 were generated. These mice remained fertile with no significant changes in sperm count or protamine expression levels. However, modifications in sperm head shape were observed. Transmission electron microscopy revealed disrupted chromatin condensation in mutant sperm, with several morphological changes and a remarkable increase in histone retention. Overall, the findings suggest that species-specific PRM1 cysteine residue positions are crucial for nuclear shape determination and histone retention in spermatozoa.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
iScience
ISSN
2589-0042
e-ISSN
2589-0042
Volume of the periodical
28
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
113102
UT code for WoS article
001550685300001
EID of the result in the Scopus database
2-s2.0-105011370909