Enhancing adipogenesis in Wharton’s jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F25%3A00641458" target="_blank" >RIV/68378041:_____/25:00641458 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/25:00638929

Result on the web

<a href="https://doi.org/10.1038/s41598-025-16867-9" target="_blank" >https://doi.org/10.1038/s41598-025-16867-9</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Enhancing adipogenesis in Wharton’s jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation

Original language description

Wharton’s Jelly multipotent mesenchymal stromal cells (WJ-MSCs) hold potential for regenerativenmedicine, particularly in soft tissue engineering. However, their adipogenic differentiation capacitynis inferior to adipose tissue-derived MSCs (AT-MSCs). This study aimed to optimize adipogenicndifferentiation for WJ-MSCs by leveraging insights from the comparative analysis of WJ- and ATMSCnlipidomic profiles. Lipidomic profiles of non-induced cells were compared, and adipogenicndifferentiation was induced with and without exogenous oleic or linoleic acid supplementation.nDifferentiation efficiency was determined based on lipid droplet formation, triglyceride (TG)ncontent quantification, and the expression of adipogenic markers. Significant differences in TGncomposition were observed, with WJ-MSCs showing higher levels of 52-carbon TGs and AT-MSCsnhaving more 56-carbon species. Both cell types had similar fatty acid (FA) profiles, with 18-carbonnFAs making up over 50%. Adding oleic acid to the differentiation medium significantly enhancednlipid droplet formation and upregulated adipogenic markers in WJ-MSCs, aligning their adipogenicncapacity more closely with AT-MSCs. In contrast, linoleic acid showed no significant benefits. Thenstudy underscores the critical role of the initial lipidomic profile in the adipogenic differentiation ofnMSCs. Supplementation with oleic acid represents a promising approach for improving adipogenicndifferentiation of WJ-MSCs and their utility in soft tissue engineering

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

30962

UT code for WoS article

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EID of the result in the Scopus database

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