Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F02%3A00191436" target="_blank" >RIV/68378050:_____/02:00191436 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Affinity chromatography of bull seminal plasma proteins on mannan-Sepharose

Original language description

The interaction of bull seminal plasma proteins and sperm with mannan was investigated using an enzyme-linked binding assay (ELBA). A high mannan-binding activity was found in the protein fraction interacting with heparin. Mannan binding to seminal plasma proteins was inhibited by D-mannose and D-fructose, but not by D-mannose-6-phosphate, D-glucose-6-phosphate, ovalbumin and ovomucoid. Mannan inhibited the binding of bovine zona pellucida glycoproteins both to bull sperm and seminal plasma proteins. Yeast mannan immobilized to divinyl sulfone-activated Sepharose was used for the isolation of mannan-binding proteins. The protein components of this fraction were identified on the basis of relative molecular mass determination and N-terminal amino acid sequencing: RNAase dimer, PDC-109 and a protein homologous to BSP-30K (relative molecular mass 14,500). The isolated proteins were characterized by a high zona pellucida binding activity.

Czech name

Afinitní chromatografie býčích seminálních proteinů na mannan-Sepharose

Czech description

Pomocí testu ELBA byly studovány interakce proteinů semenné plasmy s mannanem. Vysoká vazebná aktivita na mannan byla pozorována u proteinové frakce reagující s heparinem. Vazba na mannan byla inhibována D-mannosou a D-fruktosou, ale neměl na ni vliv D-mannosa-6-fosfát, D-glukosa-6-fosfát, ovalbumin a ovomukoid. Mannan potlačoval vazbu glykoproteinů bovinní zony pellucidy na proteiny býčího spermatu a semenné plasmy. Kvasničný mannan imobilizovaný na divinylsufonem aktivovanou Sepharosu byl využit k izolaci proteinů vázajících mannan. Na základě určení relativní molekulové hmotnosti a sekvenování aminokyselinového N-konce byly identifikovány proteinové komponenty této frakce: dimer RNAsy, PDC-90 a protein homologni s BSP-30K (o relat. molek. hmostnosti14 500). Izolované proteiny se vyznačovaly vysokou vazebnou aktivitou na zonu pellucidu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography-B

ISSN

0378-4347

e-ISSN

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Volume of the periodical

780

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

231-239

UT code for WoS article

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EID of the result in the Scopus database

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