Aggregated forms of bull seminal plasma proteins and their heparin-binding activity.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F04%3A23033208" target="_blank" >RIV/68378050:_____/04:23033208 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/04:00000371 RIV/00216208:11310/04:7032
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Aggregated forms of bull seminal plasma proteins and their heparin-binding activity.
Original language description
Heparin-binding activity of bull seminal plasma proteins was shown to be dependent on their aggregation state. Protein fraction interacting with immobilized heparin was characterized by large polydispersity in the region of relative molecular mass of 60000 ů 100 000 while that not retained on the affinity carrier was present as aggregates with relative molecular mass >100 000. Components of heparin-binding and non-heparin-binding fractions were separated by RP HPLC and analyzed by SDS electrophoresis and N-terminal sequencing. Size exclusion chromatography of whole seminal plasma and heparin-binding proteins in the presence of D-fructose (as a component of seminal plasma) showed that the region of relative molecular masses of protein associated formswas shifted to lower values. An increase of heparin-binding activity of bull proteins, as determined by ELBA, correlates with a decrease of their aggregation state. The modulation of the aggregation state of bull proteins by seminal plasm
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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