Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F03%3A23033037" target="_blank" >RIV/68378050:_____/03:23033037 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Structural basis of HIV-1 and HIV-2 protease inhibition bya monoclonal antibody.
Original language description
The murine monoclonal antibody 1696, produced by immunisation with the HIV-1 protease,inhibits the catalytic activity of the enzyme of both the HIV-1 and HIV-2 isolates, with inhibitionconstants in the low nanomolar range. This antibody cross-reacts withpeptides that include theN-terminus of the enzyme (residues 1-7), a region which is highly conserved in sequence amongdifferent viral strains and which, furthermore, is crucial for homodimerization to the activeenzymatic form.<?xml:namespace prefix = ons = 'urn:schemas-microsoft-com:office:office' />We report here two crystal structures of a recombinant single-chain Fv fragment of mAb 1696,expressed in E. coli, as a complex with a cross-reactive peptides from the HIV-1 PR and theHIV-2 PR at 2.7 ? resolution and 1.9 ? resolution respectively. On the basis of the interactionsseen in the complex three-dimensional structures, the cross-reactivity between mAb 1696 withthe HIV-1 and HIV-2 protease and their N-terminal peptides can be explai
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GV203%2F98%2FK023" target="_blank" >GV203/98/K023: Protein crystallography analyses of liganded proteinase of human immunodeficiency virus ( HIV ) for design of novel anti - AIDS compounds</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Material Structure in Chemistry, Biology, Physics and Technology.
ISBN
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ISSN
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e-ISSN
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Number of pages
2
Pages from-to
70
Publisher name
Czech and Slovak Crystallographic Association
Place of publication
Praha, Česká republika
Event location
Nové Hrady [CZ]
Event date
Mar 13, 2003
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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