Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F05%3A00000229" target="_blank" >RIV/68378050:_____/05:00000229 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/05:00021101
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
Original language description
The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the processof positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs.
Czech name
Poziční klonování genu Hybrid sterility 1: genetické mapování a evaluace dvou kandidátních genů
Czech description
Gen Hybrid sterility 1 (Hst1) ovlivňuje fertilitu hybridních samců mezi některými laboratorními kmeny (např. C57BL/10) a myší (pod)druhu Mus musculus musculus. Během pozičního klonování Hst1 genu jsme vygenerovali kontig bakteriálních artificiálních chromosomů (BAC) a vzorkovou sekvenci kandidátní oblasti ze 129S1/SvImJ kmene. Nové genetické markery nám umožnily zúžit Hst1 oblast z 580 na 360 kb. Produkty dvou genů z této oblasti, TATA-vazebný protein (Tbp) a proteasomová podjednotka beta 1 (Psmb1), akumulují během spermatogeneze. Tyto proteiny mají konzervované C-terminalní sekvence a druhově-specifické N-konce. Vyhodnotili jsme kandidaturu těchto genů na gen Hst1 alelickým sekvenováním a semikvantitativním RT PCR v reálném čase pro jejich testikulární mRNA.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biological Journal of the Linnean Society
ISSN
0024-4066
e-ISSN
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Volume of the periodical
84
Issue of the periodical within the volume
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Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
637-641
UT code for WoS article
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EID of the result in the Scopus database
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