Complete nucleotide sequence and genome analysis of bacteriophage BFK20 ? A lytic phage of the industrial producer Brevibacterium flavum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F06%3A00044701" target="_blank" >RIV/68378050:_____/06:00044701 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Complete nucleotide sequence and genome analysis of bacteriophage BFK20 ? A lytic phage of the industrial producer Brevibacterium flavum

Original language description

Total DNA genome of bacteriophage BFK20, a lytic phage of Brevibacterium flavum CCM251 ? L-lysine industrial producer ? was sequenced and analyzed. It consists of 42968 base pairs with an overall molar G+C content of 56.2%. Fifty-five potential open reading frames were identified and annotated using various bioinformatics tools. Clusters of functionally related putative genes were defined (structural, lytic, replication and regulatory). To verify the annotation of structural proteins, they were resolvedby 2D gel electrophoresis and were submitted to N-terminal amino acid sequencing. Structural proteins identified included the portal and major and minor tail proteins. Based on the overall genome sequence comparison, similarities with other known bacteriophage genomes include primarily bacteriophages from Mycobacterium spp. and some regions of Corynebacterium spp. genomes ? possible prophages. Our results support the theory that phage genomes are mosaics with respect to each other.

Czech name

Kompletní nukleotidová sekvence a genomová analýza bakteriofága BFK20, lytického fága průmyslové bakterie Brevibacterium flavum

Czech description

Sekvence a analýza kompletního genomu dvouvláknového DNA bakteriofága BFK20. Genom je dlouhý 42 968 nukleotidů s obsahem GC párů 56,2%. Identifikovali jsme 55 otevřených čtecích rámců a anotovali jsme je pomocí různých bioinformatických nástrojů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virology

ISSN

0042-6822

e-ISSN

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Volume of the periodical

348

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

57-71

UT code for WoS article

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EID of the result in the Scopus database

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