Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897567" target="_blank" >RIV/60076658:12310/18:43897567 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00498810
Result on the web
<a href="https://peerj.com/articles/5687.pdf" target="_blank" >https://peerj.com/articles/5687.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7717/peerj.5687" target="_blank" >10.7717/peerj.5687</a>
Alternative languages
Result language
angličtina
Original language name
Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages
Original language description
Next-Generation Sequencing (NGS) technologies provide unique possibilities for the comprehensive assessment of the environmental diversity of bacteriophages. Several Bacillus bacteriophages have been isolated, but very few Bacillus megaterium bacteriophages have been characterized. In this study, we describe the biological characteristics, whole genome sequences, and annotations for two new isolates of the B. megaterium bacteriophages (BM5 and BM10), which were isolated from Egyptian soil samples. Growth analyses indicated that the phages BM5 and BM10 have a shorter latent period (25 and 30 min, respectively) and a smaller burst size (103 and 117 PFU, respectively), in comparison to what is typical for Bacillus phages. The genome sizes of the phages BM5 and BM10 were 165,031 bp and 165,213 bp, respectively, with modular organization. Bioinformatic analyses of these genomes enabled the assignment of putative functions to 97 and 65 putative ORFs, respectively. Comparative analysis of the BM5 and BM10 genome structures, in conjunction with other B. megaterium bacteriophages, revealed relatively high levels of sequence and organizational identity. Both genomic comparisons and phylogenetic analyses support the conclusion that the sequenced phages (BM5 and BM10) belong to different sub-clusters (L5 and L7, respectively), within the L-cluster, and display different lifestyles (lysogenic and lytic, respectively). Moreover, sequenced phages encode proteins associated with Bacillus pathogenesis. In addition, BM5 does not contain any tRNA sequences, whereas BM10 genome codes for 17 tRNAs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/EF16_019%2F0000759" target="_blank" >EF16_019/0000759: Centre for research of pathogenicity and virulence of parasites</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PeerJ
ISSN
2167-8359
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
DEC 10 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
24
Pages from-to
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UT code for WoS article
000452726400002
EID of the result in the Scopus database
2-s2.0-85058269715