p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F09%3A00333651" target="_blank" >RIV/68378050:_____/09:00333651 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization

Original language description

RECQL4 belongs to the conserved RecQ family of DNA helicases playing important roles in the maintenance of genome stability. Although genetic alterations in RECQL4 gene are reported to be associated with three autosomal recessive disorders, the molecularrole of RECQL4 is still poorly understood. We show that RECQL4 specifically interacts with histone acetyltransferase p300 (p300 HAT) in vivo and in vitro and that p300 acetylates one or more lysine residues of RECQL4. We also report that these residueslie within a short motif of 30 aa essential for the nuclear localization of RECQL4. Acetylation of RECQL4 by p300 in vivo leads to a significant shift of a proportion of RECQL4 protein from the nucleus to the cytoplasm. This is a result of RECQL4 inability to be imported into the nucleus. Our results are the first evidence of post-translational modification of RECQL4 and suggest that acetylation of RECQL4 by p300 regulates trafficking of RECQL4 between the nucleus and the cytoplasm.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Science

ISSN

0021-9533

e-ISSN

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Volume of the periodical

122

Issue of the periodical within the volume

Pt 8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

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UT code for WoS article

000264777700023

EID of the result in the Scopus database

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