CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10285589" target="_blank" >RIV/00216208:11310/14:10285589 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/14:00439939 RIV/68378050:_____/14:00434660
Result on the web
<a href="http://dx.doi.org/10.4161/rna.29441" target="_blank" >http://dx.doi.org/10.4161/rna.29441</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4161/rna.29441" target="_blank" >10.4161/rna.29441</a>
Alternative languages
Result language
angličtina
Original language name
CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation
Original language description
Histone acetylation modulates alternative splicing of several hundred genes. Here, we tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternativeEDB exon. p300 associates with CRE sites in the promoter via the CREB transcription factor. We created mini-gene reporters driven by an artificial promoter containing CRE sites. Both deletion and mutation of the CRE site affected EDB alternative splicingin the same manner as p300 knockdown. Next we showed that p300 controls histone H4 acetylation along the FN1 gene. Consistently, p300 depletion and CRE deletion/mutation both reduced histone H4 acetylation on mini-gene reporters. Finally, we provide evidence that the effect of CRE inactivation on H4 acetylation and alternative splicing is counteracted by the inhibition of histone deacetylases. Together, these data suggest that histone acetylation could be one of the mechanisms how promo
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP305%2F12%2FG034" target="_blank" >GBP305/12/G034: Centre for RNA Biology</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RNA Biology
ISSN
1547-6286
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
865-874
UT code for WoS article
000342901600013
EID of the result in the Scopus database
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