Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F10%3A00346752" target="_blank" >RIV/68378050:_____/10:00346752 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas
Original language description
Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Virology
ISSN
0022-538X
e-ISSN
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Volume of the periodical
84
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
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UT code for WoS article
000276358000009
EID of the result in the Scopus database
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