In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F11%3A00355300" target="_blank" >RIV/68378050:_____/11:00355300 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/11:00355300 RIV/00216208:11320/11:10105570

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In vivo kinetics of U4/U6.U5 tri-snRNP formation in Cajal bodies

Original language description

The U4/U6.U5 tri-snRNP is an essential pre-mRNA splicing factor, which is assembled in a step-wise manner before each round of splicing. It was previously shown that the tri-snRNP is formed in Cajal bodies (CBs) but little is known about the dynamics ofthis process. Here we created a mathematical model of tri-snRNP assembly in CBs and used it to fit kinetics of individual snRNPs monitored by fluorescence recovery after photobleaching (FRAP). A global fitting of all kinetic data determined key reactionconstants of tri-snRNP assembly. The rates of di-snRNP and tri-snRNP assemblies are similar and ca 230 tri-snRNPs are assembled in one CB per minute. Tri-snRNP assembly is about ten-fold faster in CBs than in the surrounding nucleoplasm, which is fully consistent with the importance of CBs for snRNP formation in rapidly developing biological systems.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Biology of the Cell

ISSN

1059-1524

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

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UT code for WoS article

000287305700008

EID of the result in the Scopus database

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