Btk is a positive regulator in the TREM-1/DAP12 signaling pathway
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F11%3A00370093" target="_blank" >RIV/68378050:_____/11:00370093 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1182/blood-2010-11-317016" target="_blank" >http://dx.doi.org/10.1182/blood-2010-11-317016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1182/blood-2010-11-317016" target="_blank" >10.1182/blood-2010-11-317016</a>
Alternative languages
Result language
angličtina
Original language name
Btk is a positive regulator in the TREM-1/DAP12 signaling pathway
Original language description
TREM-1 receptor has been implicated in the production of proinflammatory cytokines and chemokines during bacterial infection and sepsis. For downstream signal transduction, TREM-1 is coupled to the ITAM-containing adaptor DAP12. We demonstrate that Bruton tyrosine kinase (Btk), becomes phosphorylated upon TREM-1 triggering. In cell lines, in which expression of Btk was diminished by shRNA-mediated knockdown, phosphorylation of Erk1/2 and PLC?1 and Ca+ mobilization were reduced after TREM-1 stimulation.TREM-1-induced production of the pro-inflammatory cytokines, TNF-? and IL-8, and up-regulation of activation/differentiation cell surface markers were impaired in Btk knockdown cells.Intact membrane localization and a functional kinase domain were required for TREM-1-mediated signaling. After TREM-1 engagement, TNF-? production by PBMCs was reduced in the patients suffering from XLA, a disease caused by mutations in the BTK gene.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1M0506" target="_blank" >1M0506: Center of Molecular and Cellular Immunology</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Blood
ISSN
0006-4971
e-ISSN
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Volume of the periodical
118
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
936-945
UT code for WoS article
000293221700020
EID of the result in the Scopus database
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