Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00381622" target="_blank" >RIV/68378050:_____/12:00381622 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1126/scisignal.2002720" target="_blank" >http://dx.doi.org/10.1126/scisignal.2002720</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/scisignal.2002720" target="_blank" >10.1126/scisignal.2002720</a>

Result language

angličtina

Original language name

Sequential inter- and intrasubunit rearrangements during activation of dimeric metabotropic glutamate receptor 1

Original language description

The metabotropic glutamate receptor 1 (mGluR1), a class C member of the heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor family, is a constitutive dimer that regulates excitatory neurotransmission. We investigated the role of homodimer formation in mGluR1 activation by examining activation-dependent inter-and intrasubunit conformational changes by fluorescence resonance energy transfer (FRET). We inserted yellow and cyan fluorescent proteins in the second intracellular loopand at the carboxyl terminus of mGluR1 to act as FRET sensors and expressed these proteins in human embryonic kidney 293 cells. Agonist-dependent activation of these mGluR1 chimeras rapidly increased the intersubunit FRET, suggesting rapid movement of the subunits relative to each other. After intersubunit movement, the intrasubunit FRET decreased, reflecting conformational changes within a subunit. Cotransfection of chimeric receptor subunits that were capable or incapable of G protein

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Science Signaling

ISSN

1937-9145

e-ISSN

—

Volume of the periodical

5

Issue of the periodical within the volume

237

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

—

UT code for WoS article

000307885200002

EID of the result in the Scopus database

—