Nuclear PIP2 and myosin I: new important players in DNA transcription

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00389003" target="_blank" >RIV/68378050:_____/12:00389003 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Nuclear PIP2 and myosin I: new important players in DNA transcription
Original language description
Nuclear myosin I (NM1) is a 120 kDa molecular motor described in the cell nucleus. NM1 was shown to be involved in chromatin remodeling, repositioning of transcriptionally activated regions and also in transcription with RNA pol I. Myosin 1C binds to negatively charged phospholipids, specifically to phosphatidylinositol-(4,5)bisphosphate (PIP2) and this binding tethers myosin I to plasma membrane. Based on this we asked if NM1 also has binding properties to PIP2. We made single-point mutations in the pleckstrin homology (PH) domain of NM1 and applied fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS). Mutant NM1 became faster in mobility compared to its wild type NM1. We then depleted PIP2 in the nucleusby co-transfecting the cells with inositol 5-phosphatase. After PIP2 depletion in nuclei, NM1 became faster showing once more that PIP2 binding reduces NM1 mobility. Phospholipase C delta (PLC?) is an enzyme which binds to PIP2 via its PH
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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