Involvement of Werner syndrome protein in MUTYH-mediated repair of oxidative DNA damage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00390248" target="_blank" >RIV/68378050:_____/12:00390248 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gks648" target="_blank" >http://dx.doi.org/10.1093/nar/gks648</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gks648" target="_blank" >10.1093/nar/gks648</a>

Result language

angličtina

Original language name

Involvement of Werner syndrome protein in MUTYH-mediated repair of oxidative DNA damage

Original language description

Reactive oxygen species constantly generated as by-products of cellular metabolism readily attack genomic DNA creating mutagenic lesions such as 7,8-dihydro-8-oxo-guanine (8-oxo-G) that promote aging. 8-oxo-G:A mispairs arising during DNA replication areeliminated by base excision repair initiated by the MutY DNA glycosylase homologue (MUTYH). Here, by using formaldehyde crosslinking in mammalian cell extracts, we demonstrate that the WRN helicase/exonuclease defective in the premature aging disorder Werner syndrome (WS) is recruited to DNA duplex containing an 8-oxo-G:A mispair in a manner dependent on DNA polymerase lambda (Pol lambda) that catalyzes accurate DNA synthesis over 8-oxo-G. Similarly, by immunofluorescence, we show that Pol lambda is required for accumulation of WRN at sites of 8-oxo-G lesions in human cells. Moreover, we show that nuclear focus formation of WRN and Pol lambda induced by oxidative stress is dependent on ongoing DNA replication and on the presence of MUT

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

8449-8459

UT code for WoS article

000309464300033

EID of the result in the Scopus database

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