In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta
Result description
DNA polymerase (pol) ? is thought to be the leading strand replicase in eukaryotes, whereas pols ? and ? are believed to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol ? is halted by an abasic site (apurinic/apyrimidinic (AP) site). In this study, we present in vitro evidence that human pols ?, ?, and ? can perform translesion synthesis (TLS) of an AP site in the presence of pol ?, likely by initiating the 3OHs created at the lesion by the arrested pol ?. However, in the case of pols ? and ?, this TLS requires the presence of a DNA gap downstream from the product synthesized by the pol ?, and the optimal gap for efficient TLS is different for the two polymerases. Collectively, our in vitro results support the existence of a mechanism of gap-directed TLS at an AP site involving a switch between the replicative pol ? and the repair pols ? and ?.
Keywords
DNA damageDNA polymeraseDNA repairDNA replicationDNA-protein interaction
The result's identifiers
Result code in IS VaVaI
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta
Original language description
DNA polymerase (pol) ? is thought to be the leading strand replicase in eukaryotes, whereas pols ? and ? are believed to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol ? is halted by an abasic site (apurinic/apyrimidinic (AP) site). In this study, we present in vitro evidence that human pols ?, ?, and ? can perform translesion synthesis (TLS) of an AP site in the presence of pol ?, likely by initiating the 3OHs created at the lesion by the arrested pol ?. However, in the case of pols ? and ?, this TLS requires the presence of a DNA gap downstream from the product synthesized by the pol ?, and the optimal gap for efficient TLS is different for the two polymerases. Collectively, our in vitro results support the existence of a mechanism of gap-directed TLS at an AP site involving a switch between the replicative pol ? and the repair pols ? and ?.
Czech name
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Czech description
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Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Volume of the periodical
286
Issue of the periodical within the volume
37
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
32094-32104
UT code for WoS article
000294726800019
EID of the result in the Scopus database
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Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
EB - Genetics and molecular biology
Year of implementation
2011