Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00392777" target="_blank" >RIV/68378050:_____/13:00392777 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M112.449231" target="_blank" >http://dx.doi.org/10.1074/jbc.M112.449231</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M112.449231" target="_blank" >10.1074/jbc.M112.449231</a>

Result language
angličtina
Original language name
Cross-talk between Tetraspanin CD9 and Transmembrane Adaptor Protein Non-T Cell Activation Linker (NTAL) in Mast Cell Activation and Chemotaxis
Original language description
Chemotaxis, a process leading to movement of cells towards increasing concentrations of chemoattractants, is essential, among others, for recruitment of mast cells within target tissues where they play an important role in innate and adaptive immunity. In this study we prepared a new mAb specific for the tetraspanin CD9. Binding of the antibody to bone marrow derived mast cells (BMMCs) triggered activation events which included cell degranulation, Ca2+ response, dephosphorylation of ezrin/radixin/moesin(ERM) family proteins and potent tyrosine phosphorylation of the non-T cell activation linker (NTAL) but only weak phosphorylation of the linker for activation of T cells (LAT). As documented by electron microscopy of isolated plasma membrane sheets, CD9 colocalized with the high-affinity IgE receptor (Fc?RI) and NTAL but not with LAT. Further tests showed that both anti-CD9 antibody and its F(ab)2 fragment inhibited mast cell chemotaxis towards antigen. Experiments with BMMCs deficient
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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